Koichiro Sumi, Kana Yamazaki, Ryuichi Nishii, Misato Sakuda, Kentaro Nakamura, Kinya Ashida, Kentaro Tamura, Tatsuya Higashi
Abstract
Although the standard method to evaluate skeletal muscle protein synthesis (MPS) is muscle biopsy, the method is invasive and problematic for multisite use. We conducted a small pilot study in volunteers to investigate changes in MPS according to skeletal muscle.
Introduction
Skeletal muscles play an important role not only in improving the performance of athletes but also in maintaining health in the general population [1–5]. Skeletal muscle protein synthesis (MPS) is essential for the growth and homeostasis of skeletal muscle [6].
Material and Methods
The protocol of this study was conducted in compliance with the 1964 Declaration of Helsinki.
Result
Seven healthy men were registered in this study, but one could not complete PET due to the urge to urinate. The data from the 6 remaining participants were included in the analysis.
Discussion
In this pilot study, we successfully demonstrated a non-invasive imaging technique for estimating MPS. Additionally, we estimated and compared the differences in MPS after ingestion of milk protein and water.
Acknowledgments
We express special thanks to all the clinical research assistants and laboratory assistants at QST and Meiji Co., and to M.R. Zhang, Ph.D. and K. Kawamura, Ph.D., who completed the timely production of [11C]Met.
Citation: Sumi K, Yamazaki K, Nishii R, Sakuda M, Nakamura K, Ashida K, et al. (2024) Unique advantages of dynamic l-[11C]methionine PET/CT for assessing the rate of skeletal muscle protein synthesis: A pilot trial in young men. PLoS ONE 19(7): e0305620. https://doi.org/10.1371/journal.pone.0305620
Editor: Juan J. Loor, University of Illinois, UNITED STATES
Received: January 24, 2024; Accepted: June 3, 2024; Published: July 30, 2024
Copyright: © 2024 Sumi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: The anonymized data that support the findings of this study are available in the Zenodo public repository with the Digital Object Identifier (DOI) “10.5281/zenodo.10993747.
Funding: The author(s) received no specific funding for this work.
Competing interests: I have read the journal’s policy and the authors of this manuscript have the following competing interests: [K. S., M. S., K. N., and K. A. are salaried employees of Meiji Co., Ltd., a food company, which also provided experimental funding, materials, facility, and staff assistance to this research. But, Meiji Co., Ltd. did not play any role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. K. Y., R. N., K. T., and T. H. has no conflicts of interest to declare.]
Source: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0305620#ack
