Meryem Baysan, Mark Broere, Maarten E. Wille, Jule E. Bergsma, Egbert G. Mik, Nicole P. Juffermans, Roula Tsonaka, Johanna G. van der Bom, Sesmu M. Arbous
Abstract
Describing mitochondrial oxygenation (mitoPO2) and its within- and between-subject variability over time after 5-aminolevulinic acid (ALA) plaster application in healthy volunteers.
Introduction
Adequate tissue and cellular oxygenation is one of the cornerstones of therapy in critically ill patients, which is guided by regular monitoring of the patient’s circulation and oxygenation [1]. Monitoring techniques used for these purposes include mean arterial pressure, lactate, venous-to-arterial carbon dioxide difference, central venous oxygen saturation, near infrared spectroscopy (NIRS) and side stream darkfield (SDF) imaging.
Materials and Methods
We performed a prospective cohort study in healthy volunteers at Leiden University Medical Center, the Netherlands. We used LUMC notice boards to recruit volunteers. Interested individuals gave written informed consent after assessment of eligibility by a trained study team member.
Result
After assessment of eligibility, 18 healthy volunteers were included in this study, 16 were analyzed (Fig 1). One healthy volunteer was excluded due to brown plaster allergy. Another healthy volunteer was excluded from the analyses, since no valid mitoPO2 measurement could be achieved during the study.
Discussion
We performed a study to describe the mitoPO2, the within- and between subject variability of the mitoPO2 values during the first 31 hours after application of the ALA plaster in healthy volunteers. The mitoPO2 was relatively stable over a period of 28 hours of ALA plaster time with a median range of 42.4–48.2 mm Hg, while there was a decline at 31 hours ALA plaster time.
Conclusion
The mitoPO2 values and within-subject variability remained relatively steady in healthy volunteers during the first 31 hours after ALA plaster application, with a median mitoPO2 of 42.4–48.2 mm Hg and within-subject variability of 6.3–11.5 mm Hg respectively.
Citation: Baysan M, Broere M, Wille ME, Bergsma JE, Mik EG, Juffermans NP, et al. (2024) Description of mitochondrial oxygen tension and its variability in healthy volunteers. PLoS ONE 19(6): e0300602. https://doi.org/10.1371/journal.pone.0300602
Editor: Gaetano Santulli, Albert Einstein College of Medicine, UNITED STATES
Received: July 20, 2023; Accepted: February 27, 2024; Published: June 3, 2024
Copyright: © 2024 Baysan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: All relevant data are within the manuscript and its Supporting Information files.
Funding: This research was financially supported by Grant PPOC-16-31 by VWS-PPOC, Amsterdam, the Netherlands. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: I have read the journal’s policy and the authors of this manuscript have the following competing interests: E.G. Mik is a founder and shareholder of Photonics Healthcare B.V. (Utrecht, The Netherlands). Photonics Healthcare develops and commercializes the COMET measuring system for mitochondrial oxygen measurements. The other authors have declared that no competing interests exist. This does not alter our adherence to PLOS ONE policies on sharing data and materials.
Source: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0300602#abstract0
